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Quantitation of soluble and membrane‐bound FC7RIIA (CD32A) mRNA in platelets and megakaryoblastic cell line (Meg‐01)
Author(s) -
Markovic Boban,
Wu Zhanhe,
Chesterman Colin N.,
Chong Beng H.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05241.x
Subject(s) - platelet , exon , messenger rna , microbiology and biotechnology , chemistry , alternative splicing , rna splicing , gene isoform , receptor , immune system , membrane glycoproteins , cell culture , glycoprotein , biology , gene , rna , immunology , biochemistry , genetics
Summary .FCγ receptors (FCγRS) are glycoproteins on platelet surface that bind IgG‐containing immune complexes. However, excessive binding of immune complexes leads to platelet activation and thrombosis or increased platelet clearance and thrombocytopenia. In this study, FC7R transcripts in platelets and megakaryoblastic cell line (Meg‐01) were investigated using specifically designed oligonucleotides and a new quantitative in situ hybridization assay. Platelets and Meg‐01 cells were found to express only FCγRII transcripts. Of FCγRIIA mRNA isoforms (FCγRIIA, B and C), FCgMRIIA mRNA predominates in these cells. Platelets and Meg‐01 cells contain both alternative spliced forms of FCγRIIA mRNA, those with and without the transmembrane (TM) exon and both forms were present in near equal amounts. In contrast, FCγRIIA transcript with the TM exon predominates in neutrophils and monocytes, suggesting that the splicing of the TM exon is under lineage‐specific control.