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Acute myeloblasts leukaemia without Philadelphia chromosome developing after interferon therapy for chronic myelocytic leukaemia with Philadelphia chromosome
Author(s) -
OHTSUKA EIICHI,
KIKUCHI HIROSHI,
ABE YUSEI,
MORTYAMA KYOKO,
OHNO EIJI,
HIROTA KIYOSHI,
TEZONO KAZUHITO,
NASU MASARU
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05223.x
Subject(s) - philadelphia chromosome , cd33 , breakpoint cluster region , trisomy 8 , cancer research , trisomy , fluorescence in situ hybridization , abl , bone marrow , interferon , chromosome , medicine , chromosome 9 , cytogenetics , biology , cd34 , immunology , chromosomal translocation , genetics , gene , stem cell , tyrosine kinase , receptor
Summary. We report a patient who developed Philadelphia chromosome negative acute myeloblastic leukaemia with trisomy 8 and trisomy 11 after receiving treatment with alkylating agents and interferon for chronic myelocytic leukaemia positive for Philadelphia chromosome. Leukaemic cells were positive for myeloperoxidase and expressed CD13, CD33 and DR; some expressed CD2, CD4 and CD34. The fluorescence in situ hybridization method revealed that bcr‐abl fusion genes were absent from > 90% of the bone marrow cells. The major bcr rearrangement was not detected by Southern blot analysis. We conclude that the leukaemic cells negative for Philadelphia chromosome may have developed as a result of treatment with alkylating agents and interferon in the present case.