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Detection of c‐fms protooncogene in early mouse embryos by whole mount in situ hybridization indicates roles for macrophages in tissue remodelling
Author(s) -
HUME DAVID A.,
MONKLEY SUSAN J.,
WAINWRIGHT BRANDON J.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05220.x
Subject(s) - in situ hybridization , embryo , in situ , microbiology and biotechnology , mount , biology , embryogenesis , chemistry , messenger rna , genetics , gene , organic chemistry , computer science , operating system
Summary. The c‐fms protooncogene which encodes the receptor for macrophage colony‐stimulating factor (CSF‐1) was localized in the developing mouse embryo by whole in situ hybridization, c‐fms was expressed first in placental trophoblasts. Around 9‐5 dpc, isolated c‐/ms‐positive cells became detectable in the yolk sac and by 10‐5 dpc large numbers were detectable throughout the embryo. The localization of c‐fms expression was consistent with its restriction to macrophages, and with the location of those macrophages in sites of tissue turnover and extensive cell death.