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The effect of interleukin‐12 in ex‐vivo expansion of human haemopoietic progenitors
Author(s) -
BERTOLINI FRANCESCO,
SOLIGO DAVIDE,
LAZZARI LORENZA,
CORSINI CHIARA,
SERVIDA FEDERICA,
SIRCHIA GIROLAMO
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05219.x
Subject(s) - progenitor cell , cytokine , cd34 , biology , interleukin 3 , microbiology and biotechnology , ex vivo , cd19 , stem cell , immunology , myeloid , interleukin 15 , stem cell factor , haematopoiesis , interleukin , t cell , in vivo , interleukin 21 , antibody , immune system
Summary. We evaluated progenitor cell proliferation in cultures supplemented by different cytokine combinations in the presence or absence of IL‐12. In cultures of low density cells, cytokine combinations including IL‐12 were associated to a greater proliferation (up to 6.7 ± 2.5 CFU‐GM fold expansion). However, in cultures of purified CD34 + cells the more efficient cytokine combination (147 ± 49 CFU‐GM fold expansion) was SCF, IL‐3, IL‐11 and MlP‐la, and the addition of IL‐12 did not further enhance expansion of progenitors. These results indicate that accessory cells, lost in CD34 + cell purification, could be in part responsible for IL‐12 effect on progenitor cell proliferation. In CD34 + cell cultures the addition of IL‐12 led to CD19 mRNA generation, suggesting that IL‐12 acts on haemopoietic cells with both myeloid and lymphoid potential.