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Differential effects of recombinant human interleukin‐13 on the in vitro growth of human haemopoietic progenitor cells
Author(s) -
Xi XIAODONG,
SCHLEGEL NICOLE,
CAEN JACQUES P.,
MINTY ADRIAN,
FOURNIER SYLVIE,
CAPUT DANIEL,
FERRARA PASCUAL,
HAN ZHONG C.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05216.x
Subject(s) - haematopoiesis , progenitor cell , interleukin 3 , in vitro , megakaryocyte , cord blood , colony stimulating factor , cytokine , peripheral blood mononuclear cell , biology , phytohaemagglutinin , granulocyte macrophage colony stimulating factor , cd34 , interleukin , microbiology and biotechnology , immunology , chemistry , stem cell , biochemistry , cytotoxic t cell , antigen presenting cell
Summary. Effects of recombinant human interleukin (IL)‐13 on in vitro haemopoiesis from non‐adherent mononuclear cells (NAMC) or highly enriched CD34 + cells of human cord blood (CB) were studied. IL‐13 significantly increased megakaryocyte (MK) colony formation from either NAMC or CD34 + cells cultured in a plasma clot system supplemented with aplastic anaemia serum (AAS) and phytohaemag‐glutinin‐stimulated human peripheral blood leucocyte‐conditioned medium (PHA‐LCM) in a dose‐dependent manner. Experiments using a modified plasma clot culture, in which normal AB serum and various cytokines were added to replace AAS and PHA‐LCM, demonstrated an increased MK colony number in the presence of IL‐13, especially in combination with IL‐3. However, IL‐13 had no stimulatory effect, but rather a slight inhibitory effect in some cases on granulocyte‐macrophage (GM) colony formation in both plasma clot cultures. Furthermore, the growth of GM progenitor cells in a methylcellulose culture system in the presence of IL‐3, GM‐CSF, Epo, G‐CSF or in combination was significantly inhibited by the addition of IL‐13. On the other hand, high concentrations (lOOng/ml) of IL‐13 were needed to cause a slight inhibition on the growth of BFU‐E‐derived colonies under the same methylcellulose culture. These results indicate that IL‐13, alone and synergistically with the effect of IL‐3, promotes MK colony formation, but it inhibits the growth of GM and erythroid progenitor cells in vitro.