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Establishment and characterization of IRTA17 and IRTA21, two novel acute non‐lymphocytic leukaemia cell lines with t(16;21) translocation
Author(s) -
HIYOSHI MOTUFUMI,
YAMANE TAKAHISA,
HIRAI MANABU,
TAGAWA SHINICHI,
HATTORI HIDEKI,
NAKAO YOSHITAKE,
YASUI YOSHINORI,
KOH KEYRYANG,
HINO MASAYUKI,
TATSUMI NORIYUKI
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05168.x
Subject(s) - chromosomal translocation , biology , karyotype , cell culture , bone marrow , cytogenetics , microbiology and biotechnology , reverse transcription polymerase chain reaction , cancer research , genetics , immunology , messenger rna , gene , chromosome
Summary. The t(16;21)(pll;q22) translocation is an infrequent chromosomal abnormality, but seems specific to acute non‐lymphocytic leukaemia (ANIL). We established two cell lines with t(16;21)(pll;q22) from the bone marrow of a patient with ANLL in relapse. Their morphological, karyotypic, immunohistochemical and genetic features are examined. Although both cell lines show monocytoid features morphologically, they express only CD13 (My 7) and CD34, and neither expressed monocytoid or lymphoid markers. Reverse transcription‐polymerase chain reaction showed that both cell lines expressed a similar TLS‐ERG chimaeric mRNA as a result of the t(16;21)(pll;q22) translocation. As far as we know, there is no report of a leukaemia cell line with t(16;21). These cell lines represent a useful tool for leukaemia research.