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Rapid and sensitive non‐radioactive assay for the detection of clonal gene rearrangements in B‐lineage acute leukaemia
Author(s) -
Martin Gerald,
Mairs Robert,
Platt Jeremy,
Gibson Brenda
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb03321.x
Subject(s) - polymerase chain reaction , microbiology and biotechnology , dna , biology , gene , gene rearrangement , gel electrophoresis , genetics
Summary. We have applied a simple and non‐radioactive assay system used in conjunction with polymerase chain reaction amplification and high‐resolution polyacrylamide gel electrophoresis to detect clonal immunoglobulin gene rearrangements in leukaemia patient DNA. This technique is as sensitive as isotopic methods, allowing the detection of clonal DNA diluted 1 in 10 000 in non‐clonal DNA.