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Anti β2 glycoprotein I antibodies: detection and association with thrombosis
Author(s) -
Martinuzzo M. E.,
Forastiero R. R.,
Carreras L. O.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb03317.x
Subject(s) - antiphospholipid syndrome , medicine , antibody , immunology , lupus anticoagulant , isotype , thrombosis , epitope , titer , population , gastroenterology , monoclonal antibody , environmental health
Summary. It has been demonstrated that antiphospholipid antibodies (aPL) recognize epitopes formed by anionic phospholipids and protein cofactors. β 2 glycoprotein I (β 2 GPI) is accepted as the cofactor of anticardiolipin antibodies (aCL). In the present study we explored the presence and clinical associations of anti β 2 GPI antibodies of IgG isotype (aβ 2 GPI‐IgG), measured by ELISA. We studied sera from 169 patients with aCL and/or lupus anticoagulant (LA), including 52 patients with systemic lupus erythematosus and 49 with primary antiphospholipid syndrome (PAPS). We found 31.9% positive sera for aβGPI‐IgG in the whole population and 48.6% in the aCL‐IgG(+) group. There was a good correlation between the titre of aCL‐IgG and the optical density for aβGPI‐IgG ( r = 0.69, P <0.01). The presence of aβ 2 GPI‐IgG was associated with the presence of aCL‐IgG ( P <0.0001) and LA ( P <0.0005). However, none of 23 LA (+) patients without aCL had aβ 2 GPI‐IgG. We found a statistically significant association between the presence of aβ 2 GPI‐IgG and a history of venous thromboembolism (VTE) in our patients ( P <0.005). This association was observed in PAPS ( P <0.05) but not in secondary antiphospholipid syndrome (SAPS). Our study confirms that some aPL(+) sera react with β 2 GPI in special experimental conditions. In addition, the presence of these antibodies is associated with a history of VTE.