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Correlations between the intensity of fMLP‐dependent respiratory burst and cellular fatty acid composition in human neutrophils
Author(s) -
Bellavite P.,
Guarini P.,
Biasi D.,
Carletto A.,
Trevisan M. T.,
Caramaschi P.,
Bambara L. M.,
Corrocher R.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb03300.x
Subject(s) - arachidonic acid , linoleic acid , palmitic acid , respiratory burst , biochemistry , chemistry , fatty acid , enzyme
Summary. The peripheral blood neutrophils were isolated from a group of normal subjects and their fatty acid composition determined by capillary gas‐chromatography. The superoxide (O 2 ) release by the same cell preparations in response to formyl‐methionyl‐leucyl‐phenylalanine was also determined following cytochrome c reduction in a microplate assay. A strong negative correlation was found between C18:2 (linoleic acid) (r = ‐0.703, P ‐0.01) and C:16:0 (palmitic acid) ( r = ‐0.569, P = 0.009) and fMLP‐stimulated O 2 release, whereas C20:4 (arachidonic acid) correlated Positively ( r = 0.448, P = 0.048). Other fatty acids, namely C12:0, C14:0, C16:1, C18:1, C18:3, C18:4, C20:0, C20:1, C20:2, C20:5, C22:0, C22:1, C22:6, C24:0 and C26:0, were not correlated with O 2 . No correlations were found between fatty acid composition and O 2 release from resting cells and from cells stimulated by phorbol‐myristate acetate. These results suggest that the fatty acid composition of blood neutrophils may be a critical factor determining the capability of releasing free radicals in response to formylpeptides. Moreover, since a concomitant increase of arachidonic acid and decrease of its precursor linoleic acid has been found in high‐responsive neutrophils, the rate of the enzymes of the arachidonic acid biosynthetic pathway (elongases and desaturases) appear to play an important role.