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A luminescene method for the quantitative determination of phagocytosis of erythrocytes, of malaria‐parasitized eryithrocytes and of malarial pigment
Author(s) -
Schwarzer Evelin,
Turrini Franco,
Arese Paolo
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb05112.x
Subject(s) - phagocytosis , pigment , malaria , biology , malarial parasites , chemistry , biochemistry , microbiology and biotechnology , plasmodium falciparum , immunology , organic chemistry
Summary. A method is described for the quantitative measurement of phagocytosis of human erythrocytes, malaria‐parasitized erythrocytes and isolated malarial pigment by adherent human monocytes. The method utilizes measurement of haem‐elicited luminescence both for the assay of ingested haemoglobin or malarial pigment haem and for the quantification of adherent monocytes. The latter is based on assay of luminescence elicited by cytochrome haem. The method utilizes the same low‐cost reagents and equipment for assay of ingested haem and for quantification of adherent monocytes. The method is fast and extremely sensitive. The lower sensitivity limit is 500 monocytes and 10 RBC. or RBC equivalents in the case of malarial pigment, per assay. A detailed protocol with full calculations of a typical phagocytosis experiment of oxidatively damaged RBC, malarial pigment and control RBS is presented.