Premium
Human platelet antigen4 (Br) genotyping by ASPA: allele‐specific primer amplification (PCR‐SSP)
Author(s) -
Simsek S.,
Bleeker P. M. M.,
Heeremans J.,
Dem Borne A. E. G. Kr.
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb05096.x
Subject(s) - primer (cosmetics) , genotyping , allele , genotype , biology , polymerase chain reaction , microbiology and biotechnology , genetics , variants of pcr , gene , chemistry , organic chemistry
Summary. We have developed a PCR assay named ASPA (allele‐specific primer amplification) to determine the HPA‐5a and −5b genotypes. It consists of two PCR‐reactions. One primer of each priomer set has a 3’‐end nucleotide which is specific for A or G at position 505 of the nHPA‐5b or −5a allele respectively. The HPA‐5 genotypes determined in this way were strictly concordant with the genotypes established by the PCR‐ASRA and with the phenotypes established using MAIPA. The ASPA is a rapid and reliable technique and can be used for the determination of alleles which code for platelet antigen allotypes.