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Interleukin‐3 is an autocrine growth factor of human megakaryoblasts, the DAMI and MEG‐01 cells
Author(s) -
Chen Yuan Zhong,
Gu Xue Fan,
Caen Jacques P.,
Han Zhong Chao
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb05063.x
Subject(s) - autocrine signalling , cell culture , k562 cells , haematopoiesis , cytokine , receptor , biology , microbiology and biotechnology , interleukin , immunology , stem cell , genetics
Summary. Interleukin‐3 (IL‐3), a cytokine konw to be produced by activated T lymphocytes, mast cells, eosinophils and neutrophils, is a potent stimulator of normal haemopoiesis, particularly magakaryocytopoiesis. However, it remains unknown whether leukaemic magakaryoblasts can produce IL‐3 and whether IL‐3 is involved in the pathological process of megakaryoblastic leukaemia. In this study, several human leukaemia cell lines with or without megakaryocytic features the DAMI, MEG‐01, HEL, K562, HL‐60 and U937, were chosen as the models. It was first demonstrated by reverse transcriptase‐polymerase chain reaction (RT‐PCR) and indirect immunofluorescence assay that IL‐3 was expressed in DAMI and MEG‐01 cells, but not in other cell lines, although two erythroleukaemic cells, the HEL and K562, also possess some megakaryocytic features. Interestingly, the mRNA for IL‐3 receptor was detected in nearly all the cell lines except K562 cells, suggesting that expression of IL‐3 and its receptor may be dissociated in most of the cell lines and that co‐expression of IL‐3 and its receptor exists in megakaryoblastic cell lines, the DAMI and MEG‐01. Of the cell lines which did not express IL‐3 under unstimulated condition, only HEL cells were able to express IL‐3 mRNA after treatment with PMA for 72 h. Furthermore, the proliferation of DAMI and MEG‐01 cells could be enhanced in the presence of IL‐3 and suppressed by the anti‐IL‐3 antibody and the IL‐3 antisense oligodexyonucleotides (ODNs). These findings indicate that IL‐3, as an autocrine growth factor, is involved in the growth of some megakaryocytic leukaemia cell lines.