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Haemopoietic colony‐forming cells from peripheral blood stem cells harvests: cytokine requirements and lineage potential
Author(s) -
Baines P.,
Truran L.,
BaileyWood R.,
Hoy T.,
Lake H.,
Ponton C. H.,
Burnett A.
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb05062.x
Subject(s) - clonogenic assay , progenitor cell , biology , stem cell , interleukin 3 , cytokine , cd34 , immunology , myeloid , haematopoiesis , granulocyte colony stimulating factor , stem cell factor , colony stimulating factor , microbiology and biotechnology , cell , t cell , interleukin 21 , chemotherapy , biochemistry , immune system , genetics
Summary. We have measured the in vitro growth requirements of progenitor cells released into the blood of cancer patients following administration of chemotherapy and cytokines. In order to distinguish the direct effects of cytokines on progenitors from those activating acessory cells, we have comparied clonogenic grwoth before and after CD34‐positive selection of progenitors, in serum‐free conditions. CD34 selection had little effect on the cytokine requirements of erythroid colony‐forming cells and single cytokines, particularly interleukin‐3, could support considerable colony growth in both mononulear and CD34+ cell suspensions. Optimal erythroid colony grwoth, however, usually required the addition of a combination of stem cells factor and interleukin‐3, in addition to erythoropoietin, which was was always required. Maximal numbers of granulocyte monocyte progenitors in mononuclear cell cultures, could be achieved with a mixture of stem cell factros, ionterleukin‐3 and granulocyte‐monocyte colony stimulating factor. How ever, after CD34 selection, full myeloid colony growth was only achieved when granulocyte colony stimulating factor was added to the above mixture. This presumably reflects loss of accessory cells, during CD34 selection, which produced this cytokine. When transplanted after 8 d of culutre. 16/22 myeloid colonies from erythorpoietin‐free cultures of peripheral blood stem cell harvests, could generate secondary multipotential. However, surface marker analysis of individual erythroid colonies revealed only the occasional presence of granulocytes and monocytes. These date demonstrate that cytokine mixtures are required for optimal colony growth, particuarly after CD34 selection, a nd that most mobilizied, blood clonogenic cells are multipotential.