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The kinetics of colony formation by CFU‐GM in vitro
Author(s) -
Lewis J. L.,
Blackett N. M.,
Gordon M. Y.
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb05052.x
Subject(s) - cfu gm , progenitor cell , in vitro , granulocyte , bone marrow , andrology , biology , immunology , kinetics , progenitor , granulocyte macrophage colony stimulating factor , microbiology and biotechnology , medicine , genetics , stem cell , physics , quantum mechanics
Summary. Video‐recordings of whole normal bone marrow granulocyte‐macrophage colony (CFU‐GM) cultures were made after 7, 14 and 21 d. Retrospective viewing of the tapes allowed the relationships to each other of the colonies scored on the three different occasions to be documented. The results show that, according to our scoring criteria, there is very little overlap between the numbers of colonies scored on days 7. 14 and 21. Moreover. only about 10% of the progenitors in a sample form day 21 colonies. The remaining progenitors form colonies earlier in the culture period and either disappear before day 21 or remain small.