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Long‐term culture and molecular biological studies highlight differences in relative BCR‐ABL expression levels in the peripheral blood and bone marrow of a patient with chronic granulocytic leukaemia
Author(s) -
Smith M. A.,
Mills K. I.,
Smith J. G.
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb05041.x
Subject(s) - peripheral blood , medicine , bone marrow , chronic myeloid leukaemia , immunology , hematology , peripheral , pathology
Summary. A patient with accelerating Ph+ve chronic granulocytic leukaemia (CGL) was considered for autologous BMT using marrow ‘purged’ by 4 weeks long‐term culture (LTC). Efficacy of purging was determined using reverse transcriptase PCR for BCR‐ABL mRNA transcripts b2a2 and b3a2. Peripheral blood and bone marrow were compared. Three observations emerged: (i) the initial b2a2:b3a2 ratios for unmanipulated blood and marrow were different with values of 9:1 and 2:1 respectively; (ii) both transcripts were successfully ‘purged’ with LTC of blood but not marrow: and (iii) LTC of marrow caused a transient increase in relative levels of b3a2 mRNA and a corresponding reduction in the b2a2 signal. This is the first case where such differences have been demonstrated in association with LTC.