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Aplasia after donor lymphocyte infusion (DLI) for CML in relapse after sex‐mismatched BMT: recovery of donor‐type haemopoiesis predicted by non‐isotopic in situ hybridization (ISH)
Author(s) -
Garicochea B.,
Rhee F. van,
Spencer A.,
Chase A.,
Lin F.,
Cross N. C. P.,
Goldman J. M.
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb05039.x
Subject(s) - aplasia , bone marrow aplasia , medicine , haematopoiesis , in situ hybridization , bone marrow , lymphocyte , donor lymphocyte infusion , immunology , cancer research , stem cell , biology , transplantation , hematopoietic stem cell transplantation , gene , genetics , gene expression
Summary. We report a patient with CML in relapse after a sex‐mismatch bone marrow transplant who was treated with donor lymphocyte infusions and developed severe marrow aplasia 3 months later. As cytogenetic analysis at this point was not possible because of the very low number of marrow cells available, we used in situ hybridization (ISH) for sex chromosomes and the BCR/ABL gene together with quantitative PCR to monitor the patient's response. The results suggest that recovering haemopoiesis was derived from donor cells and contributed to the decision not to transfuse donor marrow cells on a second occasion.