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Influence of donor lymphocytes on the incidence of primary graft failure after allogeneic bone marrow transplatition in a murine model
Author(s) -
Uharek Ltz,
Glass Bertram,
Gassmann Winfried,
Loeffler Helmut,
MuellerRchholtz Wolfgang
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04980.x
Subject(s) - bone marrow , cd8 , transplantation , immunology , spleen , medicine , cytotoxic t cell , immune system , biology , in vitro , biochemistry
Summary. We used a murine mode to determine the impact of donor lymphocyte subsets on the incidence of primary marror graft fallure after transplantation of lymphoctytedepleted bone marroe. After lethal irradiaton with 7.5Gy. Balb/c mice receivd 1 * 10 5 to 4 * 10 7 GvH‐nonreactive (C57 * Balb)F1 or GvH‐reactive C57Bl/6 marrow cells. Pretdreatment with anti‐Thy‐1,2, anti‐CD4/CD8, anti‐asialoGMl or L‐leucyl‐L‐Leucine methyld ester (Leu‐Leu‐OMe) was employed to eliminate T lymphocytes and/or natural Killer cells. Primary graft failure was defined as death with neurtophild < 0.5 * 10 9 /1. To assess longterm chimaerism, the precentage of H‐b b ‐positive spleen cells was determined. Pretreatment with anti‐Thy‐1.2, anti‐CD4/CD8 or Leu‐Leu‐OMe successfully eliminated GvHR‐induced mortality. Graft failure rates gradually declined from 88% after transplantation of 1 * 10 5 cells to 0% after transplantation of 4 * 10 7 C57B/6 cells. The incidence of graft failure, however, was not altered by T‐cell depletion, provided that the unspecific loss of marrow cells was compensated for. After transplantation of GvH‐nonreactive (C57 * Balb)Fl bone marroe, neigher ex‐viro treatment with anti‐asialo GMl nor addition of 1 * 10 7 donor thymocytes to the allograft significantly influenced engraftment. The data obtained in our animal model suggest that the total number of marrow cells in of critical importance for successful marrow engraftment and not the presence of absence to T celld, NK cells or GvHR.