Premium
Effects of 3‐5 log 10 pre‐storage leucocyte depletion on red cell storage and metabolism
Author(s) -
Heaton W. A. L.,
Holme S.,
Smith K.,
Brecher M. E.,
Pineda A.,
AuBuchon J. P.,
Nelson E.
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04923.x
Subject(s) - red cell , phlebotomy , chemistry , filtration (mathematics) , erythrocyte fragility , haemolysis , blood product , red blood cell , andrology , biochemistry , chromatography , zoology , biology , hemolysis , medicine , surgery , immunology , statistics , mathematics
SUMMARY. A new, in‐line high‐efficiency 3‐5 log 10 leucodepletion filter system (Leukotrap° RC system) was used to investigate the effect of pre‐storage white cell removal on the quality of AS‐3 red cell concentrates stored for 42 d at 4°. Median residual white cell content was 4 × 10 5 when filtration was performed at 22° within 8 h of phlebotomy ( n = 20) and 3.2 × 10 4 when filtration was performed at 4° 12‐24 h after phlebotomy ( n = 24). None exceeded 1 × 10 6 WBC per red cell product. Filtration was rapid (median 28 min), and red cell loss averaged (mean ± 1 SD) 6.4 ± 0.7%. In a paired study design, post‐transfusion recoveries of 42 d stored red cells in the filtered units averaged 84 ± 6% v 82 ± 8% for unfiltered units ( P < 0.05) and post‐storage haemolysis, ATP, osmotic fragility, K+ and pH were significantly ( P < 0.05) better in the filtered units. Reduced glycolytic activity was also observed in the filtered units, and there was a correlation between osmotic fragility, glucose consumption, and lactate produced in standard units that was not present in leucodepleted units. In conclusion, this study suggests that leucodepletion of AS‐3 red cell concentrates prior to storage results in better maintenance of the integrity of the red cell membrane with reduced glycolytic activity. There was a modest improvement in post‐infusion viability sufficient to offset the filtration‐induced loss and to result in an equivalent red cell product.