Premium
Establishment and characterization of a novel myeloid cell line from the bone marrow of a patient with the myelodysplastic syndrome
Author(s) -
Tohyama Kaoru,
Tsutani Hiroshi,
Ueda Takanori,
Nakamura Toru,
Yoshida Yataro
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04904.x
Subject(s) - myeloid , bone marrow , cell culture , myelodysplastic syndromes , cancer research , biology , mutation , immunology , medicine , genetics , gene
SUMMARY. A novel long‐term cultured myeloid cell line was established from the bone marrow of a patient with myelodysplastic syndrome (MDS). This cell line, designated MDS92. proliferated in the presence of interleukin‐3 or granulocyte‐macrophage colony‐stimulating factor and transiently in the presence of Steel factor, with a tendency for gradual maturation, and formed myeloid colonies in the semi‐solid culture condition. In addition, the MDS92 cell line represented rather complicated karyotypic abnormalities including the deletion of fifth and seventh chromosomes and a point mutation at codon 12 of the N‐ras oncogene. These characteristics of the MDS92 cell line are exclusively compatible with the property of preleukaemia.