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Pure red cell aplasia: further evidence of T cell clonal disorder
Author(s) -
Maung Zor T.,
Norden Jean,
Middleton Peter G.,
Jack Fergus R.,
Chandler John E.
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04890.x
Subject(s) - polymerase chain reaction , t cell receptor , southern blot , microbiology and biotechnology , gene , biology , gene rearrangement , cloning (programming) , t cell , genetics , immune system , computer science , programming language
Summary. We report a patient with pure red cell aplasia and type I autoimmune polyglandular syndrome who also had an expansion of suppressor T lymphocytes in the peripheral blood. Southern blotting of DNA from these cells suggested T cell receptor (TCR) γ gene rearrangement. We confirmed true clonality of this by amplification of the gene rearrangement using the polymerase chain reaction and subsequent analysis of the product by gene cloning and DNA sequencing.