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Establishment of a human T‐cell line with deficient surface expression of glycosylphosphatidylinositol (GPI)‐anchored proteins from a patient with paroxysmal nocturnal haemoglobinuria
Author(s) -
Masuda Tetsuya,
Yonemura Yuji,
Fujimoto Koji,
Hidaka Michihiro,
Nagakura Shoichi,
Nakakuma Hideki,
Hata Hiroyuki,
Sanada Isao,
Kawakita Makoto,
Takatsuki Kiyoshi
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04865.x
Subject(s) - clone (java method) , cd59 , biology , microbiology and biotechnology , decay accelerating factor , cd19 , il 2 receptor , paroxysmal nocturnal hemoglobinuria , cell culture , t cell , cd8 , virology , immunology , antigen , complement system , gene , antibody , flow cytometry , genetics , immune system
Summary A novel interleukin‐2 dependent T‐cell line, PMT‐2Y, was established from the peripheral blood of a patient with paroxysmal nocturnal haemoglobinuria (PNH) by human T lymphotropic virus type I (HTLV‐1)‐mediated transformation. PMT‐2Y cells are positive for CD2, CD3, CD4, CD25, T cell receptor αβ and HLA‐DR, but negative for CD1, CD7, CD8, CD19 and CD20, indicating that the clone belongs to a helper/inducer subset of T cells. PMT‐2Y cells have the monoclonal integration of HTLV‐I proviral DNA, suggesting that they derived from a single clone. Moreover, they lack surface expression of complement regulatory proteins such as DAF (CD55) and CD59, that are the most important glycosylphosphatidylinositol (GPI)‐anchored membrane proteins defective in haemopoietic cells of patients with PNH. Northern blot analysis, however, revealed the production of normal levels of DAF mRNAs. Thus, PMT‐2Y is derived from a PNH T cell clone and may be a useful model to study PNH.