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Phenotypic, functional and molecular analysis of CD3 − LGL expansions indicates a relationship to two different CD3 − normal counterparts
Author(s) -
Cantoni Claudia,
Totero Daniela,
Lauria Francesco,
Raspadori Donatella,
Conte Romana,
Ferrini Silvano,
Tazzari Pier Luigi,
Biassoni Roberto
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04823.x
Subject(s) - cd3 , cd8 , t cell receptor , biology , cd16 , microbiology and biotechnology , antigen , t lymphocyte , messenger rna , lymphocyte , k562 cells , immunology , t cell , gene , immune system , genetics , leukemia
Summary. In this study we have analysed the CD3 and TCR transcript expression in three CD3 − large granular lymphocyte (LGL) expansions. These LGL populations show a heterogenous pattern of expression for CD2, CD8, CD16, CD56 and CD57 antigens. LGL1 is CD2 + CD8 − CD16 + CD56 + CD57 + , while LGL2 is CD2 − CD8 + CD16 − CD56 − CD57 − ; LGL3 is similar to LGL1, except for CD8 antigen expression. Functional analysis has revealed a different behaviour of these LGL expansions in a cytotoxicity assay against the NK‐sensitive K562 cell line. LGL1 and 3 display a significant NK‐like activity, while LGL2 is inefficient against K562 target cells. TCR and CD3 transcript characterization of LGL expansions 1 and 3 showed that they expressed multiple TCRδ transcripts, a nonfunctional TCRβ transcript, CD3‐ and ‐ mRNA, but they lacked CD3δ transcript and they lacked or expressed at very low levels of CD3γ transcript. On the other hand. LGL2 expressed TCRδ, CD3‐γ, ‐ and ‐ transcripts, while it lacked CD3δ mRNA. On the basis of these data, LGL1 and 3 seem to be closely related to peripheral blood mature natural killer (NK) cells, whereas LGL2 displays a pattern of TCR and CD3 expression similar to that found in CD1 − 2 − 3 − 4 − 8 − 16 − thymocytes.