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Recombinant interferon‐γ inhibits the in vitro proliferation of human myeloma cells
Author(s) -
Palumbo Antonio,
Battaglio Silvano,
Napoli Patrizia,
Omedè Paola,
Fusaro Antonella,
Bruno Benedetto,
Boccadoro Mario,
Pileri Alessandro
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04821.x
Subject(s) - secretion , recombinant dna , interferon , stimulation , antibody , in vitro , medicine , endocrinology , dexamethasone , microbiology and biotechnology , biology , chemistry , immunology , biochemistry , gene
Summary. Interferon‐α (IFN‐α), interferon‐γ (IFN‐γ) and dexamethasone (DEX) have shown anti‐tumour effects in multiple myeloma (MM) cells. Bone marrow plasma cells from 39 MM patients were cultured to clarify the intensity and specific activity of each compound on bromo‐deoxyuridine (BrdUrd) uptake and immunoglobulin (Ig) secretion. BrdUrd uptake was inhibited by recombinant human IFN‐γ (100 U/ml) and by DEX (10 −6 m ). The stimulation index (StI), i.e. labelling index (LI) of treated samples/controls, was 0·49 0·009 (mean standard error of the mean, MSEM), P =0·0003, and 0·52·0·07 (MSEM), P <0·0001, respectively. Ig secretion was reduced by IFN‐α (100 U/ml) and DEX. The secretion index (SI), i.e. Ig quantitation of treated samples/controls, was 0·04 (MSEM), P <0·0001, and 0·52·0·04 (MSEM), P <0·0001, respectively. Finally, IFN‐γ inhibits BrdUrd uptake only and IFN‐α secretion only. In 18 patients the simultaneous addition of IFN‐α plus IFN‐γ mainly paralleled the effect of IFN‐γ on BrdUrd uptake and IFN‐α on secretion, but did not result in any additive or synergistic effect, though both BrdUrd uptake and Ig secretion were decreased to about the same extent as with DEX. These data indicate that the combination of IFN‐α plus IFN‐γ and DEX are the strongest inhibitors of both BrdUrd uptake and secretion. Since IFN‐α and IFN‐γ appear to have a different mechanism of action, their combined use could be considered as a possible new treatment strategy.