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Cytokine activity after allogeneic bone marrow transplantation, V. Analysis of IL‐2 and IFN production by isolated CD4 + and CD8 + cells
Author(s) -
Cooley Margaret A.,
Wright Louise,
Atkinson Kerry
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04818.x
Subject(s) - cytokine , transplantation , bone marrow , immunology , cd8 , bone marrow transplantation , cytotoxic t cell , medicine , microbiology and biotechnology , biology , immune system , in vitro , genetics
Summary. Previous studies from this laboratory have shown that PBMC from recipients of an HLA‐identical sibling bone marrow transplant produce levels of IL‐2 which are 10–100‐fold lower than those produced by the same number of PBMC from healthy controls, whereas production of IFN‐γ is normal. The present study examined IL‐2 and IFN production over a range of cell numbers for PBMC and for isolated CD4 + and CD8 + cells for controls and marrow transplant recipients. There was a 5‐fold lower IL‐2 production in marrow transplant recipient CD8 + cells compared with equivalent numbers of control cells, whereas no difference was found in IL‐2 production by CD4 + cells. In contrast, IFN production by CD4 + cells from marrow transplant recipients was 4‐fold higher than in controls, whereas CD8 + cells from both populations produced similar amounts of IFN. When the observed production of cytokine by PBMC was compared with the expected production based on the CD4 + and CD8 + content of the PBMC, control values were similar, but the expected values for both cytokines were approximately 2‐fold higher than the observed values for marrow transplant recipients. The results suggest that the capacity of T cells from marrow transplant recipients to produce IL‐2 and IFN is not impaired, but that the frequency of cytokine‐producing cells may be reduced, and that a negative interaction present in recipient PBMC, eliminated by isolating T‐cell subsets, is responsible for the observed low levels of cytokine production.