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Increased in vitro neutrophil adherence in a case of chronic idiopathic neutropenia
Author(s) -
Biasi Domenico,
Bambara Lisa Maria,
Carletto Antonio,
Caramaschi Paola,
Ortolani Riccardo,
Perona Giuseppe,
Bellavite Paolo
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04759.x
Subject(s) - neutropenia , adhesion , medicine , immunology , in vitro , monoclonal antibody , integrin , leukocyte adhesion deficiency , cd18 , antibody , chemistry , chemotherapy , receptor , biochemistry , organic chemistry
Summary In this report we describe a patient with persistent neutropenia whose neutrophils showed increased adhesion in a microplate assay. In three separate assays, from 12·5% to 13·7% of the patient's blood neutrophils exhibited spontaneous (unstimulated) adhesion to fetal bovine serum‐coated microplate wells, much higher than adhesion of cells from healthy controls (1·9% 2·5 SD, n = 20). The difference of spontaneous adhesion between the patient's and control neutrophils was even higher when cells from a skin‐window exudate were examined (patient: 42·1–100% adhesion: control: 3·6% 3·5 SD, n = 20). Over 80% inhibition of the increased adhesion was produced by the 60.3 anti‐CD 18 monoclonal antibody, suggesting an involvement of β2‐integrins.