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Characterization of haemolyser‐resistant cells increased in the blood of erythropoietin‐treated mice
Author(s) -
Said Ahmed A.,
Yamaguchi Teruhide,
Uchida Eriko,
Hayakawa Takao
Publication year - 1994
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1994.tb04740.x
Subject(s) - glycophorin , erythropoietin , erythropoietin receptor , transferrin receptor , transferrin , in vivo , receptor , medicine , immunology , biology , antigen , microbiology and biotechnology
Summary Recently, we reported a new in vivo assay method for erythropoietin (Epo) by means of counts of the number of haemolyser‐resistant cells (HRCs) increased in Epo‐injected mice. Here, we attempted to characterize the HRCs. Flow‐cytometric studies revealed that HRCs obtained from Epo‐injected mice expressed the transferrin receptor on their surface membranes. Furthermore, a fluorophotometric study suggested that the number of transferrin receptor‐positive cells increased in a dose‐dependent manner in response to treatment with Epo. On the other hand, flowcytometric and fluorophotometric studies of glycophorin A on HRCs using a rabbit antiglycophorin A antibody also showed a high expression of glycophorin A on them as compared with on HRCs from vehicle‐treated animals (control). The results indicated that HRCs could be defined by their expression of both transferrin receptors and glycophorin A. We concluded that HRCs might be immature reticulocytes.