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Different membrane expression of CD11b and CD 14 on blood neutrophils following in vivo administration of myeloid growth factors
Author(s) -
Hansen Per Boye,
Kjærsgaard Erik,
Johnsen Hans E.,
Gram Jesper,
Pedersen Michael,
Nikolajsen Kirsten,
Hansen Niels Ebbe
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb08644.x
Subject(s) - cd14 , granulocyte macrophage colony stimulating factor , granulocyte , myeloid , granulocyte colony stimulating factor , integrin alpha m , neutropenia , medicine , immunology , lipopolysaccharide , biology , endocrinology , flow cytometry , cytokine , toxicity , chemotherapy
Summary. During the administration of recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) or granulocyte‐macrophage CSF (rhGM‐CSF) we studied the early and late changes of membrane antigen density on neutrophils. RhG‐CSF and rhGM‐CSF both caused an early transient reduction in blood neutrophilic granulocyte‐concentration within the first 30 min after treatment followed by a marked later increase during the subsequent 24 h. During the early neutropenia quantitative flow cytometry showed an associated marked increase in the density of membrane CD11b from 169 × 10 3 before to 568 × 10 3 A.U. per cell induced by rhGM‐CSF but a non‐significant change by rhG‐CSF, suggesting that different mechanisms may be responsible for the transient neutropenia. The subsequent neutrophil granulocytosis was followed by a significantly (P<0.05) increased density of the CD14 antigen from 6.1 × 10 3 before to 15.9 × 10 3 A.U. per cell during treatment with rhG‐CSF. but not by rhGM‐CSF administration. These results demonstrate that the two cytokines may affect the function of neutrophilic granulocytes in different ways. The increased expression of CD1 1b could explain some of the side‐effects during treatment with rhGM‐CSF. The upregulation of CD14 induced by rhG‐CSF may be clinically relevant, as CD14 is an opsonic receptor for lipopolysaccharide binding proteins, acting in the defence against Gramnegative bacterial infections.