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Microenvironmentally dependent effects on murine haemopoiesis by a prolonged interleukin‐1 treatment
Author(s) -
Haan G. de,
Dontje B.,
Loeffler M.,
Nijhof W.
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb08639.x
Subject(s) - spleen , erythropoiesis , haematopoiesis , erythropoietin , bone marrow , in vivo , progenitor cell , cfu gm , immunology , stimulation , biology , medicine , endocrinology , splenocyte , interleukin 3 , andrology , stem cell , anemia , t cell , immune system , microbiology and biotechnology , antigen presenting cell
Summary. We administered recombinant human IL‐lβ (400 ng/d, s.c.) for 10 d to normal C57B1 mice and determined daily granuloid and erythroid parameters in marrow, spleen and blood. In the marrow CFU‐GM numbers were not affected but later granuloid cell stages were moderately enhanced (170%). In the spleen, however, CFU‐GM numbers were sharply increased (1600%). whereas the granuloid precursors only doubled. Blood granulocytes increased transiently to 275% on day 5. In the marrow all erythroid parameters were severely reduced. This reduction was partially compensated by the spleen where initially only BFU‐E and with some delay also more mature erythroid cells accumulated. At the end of the treatment mice were slightly anaemic. When mice were treated with IL‐1 and erythropoietin (10 U/d) simultaneously, the inhibitory effects on erythropoiesis were less severe. In agreement with in vivo results, IL‐1 inhibited in vitro colony growth of CFU‐E from normal bone marrow and spleen but spleen CFU‐E from 5 d IL‐1 treated mice were insensitive. We conclude that IL‐1 can induce stimulation or inhibition of haemopoietic progenitor cells depending on their micro‐environment.