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Ultraviolet‐B irradiation of platelets induces a dose‐dependent increase in the expression of platelet activation markers with storage
Author(s) -
Grijzenhout M. A.,
AartsRiemens M. I.,
Akkerman J. W. N.,
Nieuwenhuis H. K.,
Weelden H. van,
Prooijen H. C. van
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb04701.x
Subject(s) - platelet , cd63 , sensitization , chemistry , platelet activation , irradiation , platelet transfusion , andrology , immunology , pharmacology , biochemistry , biology , medicine , microrna , physics , microvesicles , nuclear physics , gene
Summary. Ultraviolet B (UVB) irradiation of platelet concentrates (PCs) has been proposed as a novel technology to prevent HLA sensitization. We have recently reported that 2 J/cm 2 of UVB radiation abolishes alloreactive lymphocyte responses in vitro. In order to increase the efficacy of UV irradiation for the prevention of HLA sensitization, we exposed PCs to 4 or 8 J/cm 2 of UVB and evaluated the effect of UV radiation on platelet integrity during storage. We report here that UV exposed platelets show a progressive increase in the expression of activation markers P‐selectin (GMP‐140; CD62) and LIMP‐CD63 (GP‐53; CD63) on the platelet membrane over time in a dose‐dependent manner compared to age‐matched controls. Platelet metabolism was also enhanced as evidenced by significant changes in lactate and pH during post‐irradiation storage. Based on these findings we transfused PCs within 4 h after UV irradiation. PCs exposed to 4 J/cm 2 showed normal post‐transfusion recoveries and haemostatic functions, while poor platelet recoveries were found after administration of PCs exposed to 8 J/cm 2 . We hypothesize that the rapid expression of P‐selectin on platelets exposed to the higher dose of UVB leads to an increased binding of these platelets to leucocytes in the circulation resulting in poor platelet recoveries.