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Effect of different human immunodeficiency virus type‐1 (HIV‐1) isolates on long‐term bone marrow haemopoiesis
Author(s) -
Cen Dazhi,
Zauli Giorgio,
Szarnicki Robert,
Davis Brian R.
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb03353.x
Subject(s) - haematopoiesis , human immunodeficiency virus (hiv) , bone marrow , virology , medicine , immunology , human bone , biology , stem cell , genetics , in vitro
Summary. Haemopoietic cytopenias are a frequent occurrence in human immunodeficiency virus type‐1 (HIV‐1) induced disease. In order to examine the possible direct inhibition of marrow haemopoiesis by HIV‐1, we have investigated the effect of HIV‐1 infection on myelopoiesis in long‐term bone marrow cultures. In vitro exposure of normal marrow cultures to three different lymphocytotropic HIV‐1 isolates resulted in productive infection, as demonstrated by a progressive increase of gag p24 antigen. In these experiments, ICR‐3 isolate, but not LAV' or NL4–3 isolates, accelerated the loss of non‐adherent cells. A differential ability of these HIV‐1 isolates to suppress myelopoiesis was confirmed in long‐term cultures in which virus was added continuously. In these cultures, ICR‐3, and to a lesser extent also NL4–3, but not LAV', induced a progressive decrease in the number of total non‐adherent cells as well as non‐adherent colony forming units‐granulocyte/macrophage (CFU‐GM). Furthermore, exposure of normal purified CD34 + cells to ICR‐3 induced defects in their ability to form haemopoietic colonies: this inhibitory effect was significantly relieved by pretreatment of ICR‐3 with an anti‐gp 120 antibody. Similar exposure of CD34 + cells to LAV' and NL4–3 induced no such defects. These data indicate that some HIV‐1 isolates can impair bone marrow haemopoiesis in a dose‐dependent fashion, acting, at least in part, at the level of haemopoietic stem/ progenitor cells.