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The effects of saline solutions on red cell shape: a scanning‐electron‐microscope‐based study
Author(s) -
Simpson L. O.
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb03237.x
Subject(s) - scanning electron microscope , glutaraldehyde , saline , electron microscope , red blood cell , physiological saline , chemistry , biophysics , in vitro , biomedical engineering , materials science , chromatography , biology , optics , medicine , biochemistry , endocrinology , physics , composite material
Summary Red cells appear to change shape in response to alterations in their environment both in vitro and in viva To investigate the qualitative aspects of this phenomenon, five drops of a venous blood sample were fixed in buffered glutaraldehyde for baseline observations and 0.5 ml of blood added to 4 ml of four different saline solutions. Triplicated 10 drop samples from the suspensions were fixed in the glutaraldehyde solution after 2.5, 5, 10, 20 and 40 min and prepared for scanning electron microscopy. Red cell shape analysis of the resulting micrographs showed that the cells had changed shape, although no two patterns of change were the same.