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The effects of interleukin‐8 on neutrophil fMetLeuPhe receptors, CD11b expression and metabolic activity, in comparison and combination with other cytokines
Author(s) -
Roberts Pamela J.,
Pizzey Arnold R.,
Khwaja Asim,
Carver Julia E.,
MireSluis Anthony R.,
Linch David C.
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb03132.x
Subject(s) - respiratory burst , priming (agriculture) , receptor , cytokine , endocrinology , n formylmethionine leucyl phenylalanine , chemotaxis , medicine , immunology , integrin alpha m , chemistry , biology , botany , germination
Summary. The effect of the chemotactic cytokine, IL‐8, on neutrophil function was compared with that of other cytokines, GM‐CSF, G‐CSF TNFα and IFN‐γ. IL‐8 rapidly stimulated a three‐fold enhancement of the fMLP‐stimulated respiratory burst, but this priming effect was transient compared with the slower and sustained effects of GM‐CSF and IFNγ. Apart from G‐CSF, IL‐8 was the weakest priming agent and was weaker than GM‐CSF in priming arachidonic acid metabolism stimulated by calcium ionophore. When incubated in combination, IL‐8 and TNFα were highly synergistic in their effects on respiratory burst priming, whereas IL‐8 and GM‐CSF showed little synergy. In contrast, IL‐8 was as potent as GM‐CSF at increasing the expression of neutrophil chemotactic peptide receptors and the β 2 integrin, CD11b. The latter was maximally upregulated within 5 min of stimulation with IL‐8, whereas the effect of GM‐CSF was much slower. The kinetics of neutrophil respiratory burst priming by IL‐8 were the same when measured in whole blood samples and in purified cell suspensions, and IL‐8 dose‐response curves were similar, showing that the low affinity IL‐8 receptors on erythrocytes do not rapidly sequester circulating IL‐8. The data suggest that IL‐8 plays a minor role in priming neutrophil function and that a more major activity is the regulation of neutrophil adhesion and migration.