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Granulocyte colony‐stimulating factor (G‐CSF)‐induced mobilization of circulating haemopoietic stem cells
Author(s) -
Teshima Takanori,
Harada Mine,
Takamatsu Yasushi,
Makino Kazuyoshi,
Inaba Shoichi,
Akashi Koichi,
Kondo Seiji,
Tanaka Takeshi,
Ishii Eiichi,
Niho Yoshiyuki
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb03129.x
Subject(s) - cytotoxic t cell , haematopoiesis , granulocyte colony stimulating factor , leukapheresis , stem cell , progenitor cell , immunology , mobilization , colony stimulating factor , cancer research , biology , chemotherapy , medicine , cd34 , in vitro , microbiology and biotechnology , biochemistry , history , archaeology
Summary. We studied the utility of G‐CSF for harvesting circulating haematopoietic stem cells in patients with leukaemia or lymphoma based on a comparative study in a single patient. Two successive cycles of leukapheresis following cytotoxic chemotherapy were performed in 22 patients as follows: the first cycle was performed with cytotoxic mobilization in all patients while the second cycle was randomized into two groups: cytotoxic ( n = 10) and cytotoxic plus G‐CSF (cytotoxic/G‐CSF) ( n = 12) mobilization. Repetitive cytotoxic mobilization did not alter the yields of mononuclear cells (MNC), myeloid (CFU‐GM), and erythroid (BFU‐E) progenitors. In contrast, cytotoxic/G‐CSF mobilization produced significantly higher yields of MNC (2·6‐fold), CFU‐GM (5·5‐fold), and BFU‐E (3·9‐fold) than did cytotoxic mobilization alone ( P <0·01). The ratio of CFU‐GM to BFU‐E was not affected by G‐CSF. Furthermore, G‐CSF led to an earlier peak of CFU‐GM following chemotherapy. G‐CSF is thus effective in expanding the pool of circulating haematopoietic progenitors.