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Effect of cytotoxic drugs on mature neutrophil function in the presence and absence of granulocyte‐macrophage colony‐stimulating factor
Author(s) -
Humphreys John M.,
Stringer Rebecca E.,
Hart C. Anthony,
Edwards Steven W.
Publication year - 1993
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1993.tb03071.x
Subject(s) - incubation , granulocyte , daunorubicin , cytotoxic t cell , myeloperoxidase , cyclophosphamide , n formylmethionine leucyl phenylalanine , pharmacology , prednisolone , superoxide , chemistry , vincristine , medicine , neutrophile , immunology , endocrinology , biology , biochemistry , in vitro , leukemia , inflammation , chemotherapy , enzyme
Summary. The effects of the cytotoxic drugs, adriamycin, cyclophosphamide, daunomycin (daunorubicin), prednisolone, actinomycin D. azacytidine and vincristine at concentrations of 1 μM on mature neutrophil function were examined. Up to 5 h incubation with adriamycin, azacytidine, cyclophosphamide, daunomycin and prednisolone had no effect on either luminol chemiluminescence or superoxide secretion. However, after 15 min or 1 h (but not 5 h) incubation vincristine enhanced fMet‐Leu‐Phe stimulated chemiluminescence, whilst after 5 h incubation with actinomycin D the ability of neutrophils to generate reactive oxidants in response to all stimuli tested was impaired: after 5 h incubation with adriamycin reactive oxidant production was also impaired, but only when fMet‐Leu‐Phe was used as stimulant. All of the drugs tested except azacytidine inhibited neutrophil oxidant production after 5 h incubation in the presence of granulocyte‐macrophage colony‐stimulating factor (GM‐CSF). Actinomycin D and cyclophosphamide also inhibited GM‐CSF stimulated protein biosynthesis. These data indicate that cytotoxic drugs may compromise the potentially beneficial effects of CSFs on mature neutrophil function during therapy.