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Ultraviolet irradiation modulates MHC‐alloreactive cytotoxic T‐cell precursors involved in the onset of graft‐versus‐host disease
Author(s) -
Prooijen H. C.,
AartsRiemens M. I.,
Grijzenhout M. A.,
Weelden H. van
Publication year - 1992
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1992.tb08174.x
Subject(s) - cytotoxic t cell , sensitization , phytohaemagglutinin , immunology , graft versus host disease , mixed lymphocyte reaction , t cell , human leukocyte antigen , cytotoxicity , t lymphocyte , lymphocyte , immune system , medicine , disease , antigen , biology , pathology , in vitro , biochemistry
Ultraviolet B (UVB) irradiation of cellular blood components has been proposed as a new technology to prevent HLA sensitization in recipients. Earlier studies have shown that a dose of 2 J/cm 2 abrogates the ability of lymphocytes to serve as stimulators in mixed lymphocyte cultures (MLC). In this study we have evaluated the effect of UV energy on T‐lymphocytes for the prevention of transfusion‐associated graft‐versus‐host disease (TA‐GvHD). The response of cytotoxic T‐lymphocyte precursors against host alloantigens was almost undetectable at a dose of 0·5 J/cm 2 . T‐cell proliferation in MLC or in response to phytohaemagglutinin was inhibited by more than 95% at doses of 1 J/cm 2 or higher. The data suggest that UV irradiation can be used to prevent both HLA sensitization and TA‐GvHD in recipients.