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Myelodysplastic syndrome (MDS)‐associated inhibitory activity on haematopoietic progenitor cells: contribution of monocyte‐derived lipid containing macrophages (MDLM)
Author(s) -
Ohmori Masami,
Ueda Yasunori,
Masutani Hiroshi,
Hirama Toshiyasu,
Anzai Naoyuki,
Yoshida Yataro,
Okuma Minoru
Publication year - 1992
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1992.tb08173.x
Subject(s) - polyclonal antibodies , haematopoiesis , progenitor cell , microbiology and biotechnology , monocyte , monoclonal antibody , granulopoiesis , ferritin , myelodysplastic syndromes , biology , bone marrow , myelopoiesis , macrophage , immunology , antibody , in vitro , stem cell , biochemistry
We studied myelodysplastic syndrome‐associated inhibitory activity (MDS‐IA), which inhibited colony formation in vitro of normal granulocyte‐macrophage progenitors (CFU‐GM). When adherent marrow cells were incubated with fetal calf serum for 21–24 d. monocyte‐derived lipid containing huge macrophages (MDLM) developed. MDLM from MDS marrow (MDS‐MDLMs) and their conditioned medium (MDLM‐CM) consistently suppressed the growth of normal CFU‐GM colony formation. MDS‐IA was active on CFU‐GM during the S‐phase and relatively resistant to heating. Monoclonal antibody against H subunit (acidic) ferritin and polyclonal antibody against placental ferritin neutralized the inhibitory activity of MDS‐MDLMs. In addition, cell lysates of MDS‐MDLMs reacted to both monoclonal anti‐H subunit ferritin and polyclonal anti‐placental ferritin in Western blotting analysis, indicating that the inhibitory activity was predominantly acidic isoferritin. On the other hand, MDLMs obtained from normal bone marrow had a CFU‐GM enhancing activity. These results suggest that MDS‐MDLMs may be responsible for the suppression of granulopoiesis in patients with MDS and that the suppression may be mediated by soluble factors, notably H subunit isoferritin.