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Molecular analysis of clonality in plasma cell dyscrasias
Author(s) -
Miglino Maurizio,
Gaetani Gian Franco,
Canepa Letizia,
Meloni Tullio,
Forteleoni Gavino,
Ferraris Anna Maria
Publication year - 1992
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1992.tb08164.x
Subject(s) - dyscrasia , plasma cell , paraproteinemias , medicine , immunology , multiple myeloma , antibody , monoclonal , monoclonal antibody
It has been suggested that multiple myeloma, generally considered a neoplastic disorder of mature plasma cells, may arise from a pluripotent haemopoietic stem cell. The possibility that circulating lymphocytes derive from the same neoplastic progenitor has been tested in a large number of studies in the past few years, as proof of the interest that this subject is raising among scientists, and also of its elusiveness. We studied a group of 29 patients with plasma cell dyscrasias in order to evaluate clonality of haemopoietic cell populations. The X‐linked markers hypoxantine phosphoribosyltransferase (HPRT) and phosphoglycerate kinase (PGK) disclosed no monoclonal component in seven heterozygous women. Analysis of immunoglobulin gene rearrangement with four probes showed a germline configuration in samples from 25/29 patients. Only four bone marrow samples from subjects with aggressive disease had rearranged Cμ sequence; one had rearrangement of JH and Cμ.