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Granulocytic differentiation of the human myelomonocytic leukaemia cell line ME‐1 in serum‐free culture
Author(s) -
Yanagisawa Kohsuke,
Hasegawa Hitoshi,
Fujita Shigeru
Publication year - 1992
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1992.tb08135.x
Subject(s) - cell culture , acute myelomonocytic leukemia , cellular differentiation , biology , granulocyte , microbiology and biotechnology , macrophage , immunology , leukemia , biochemistry , in vitro , gene , genetics
Summary. We have recently established ME‐1, a human myelomonocytic leukaemia cell line derived from acute myelomonocytic leukaemia with eosinophilia (M 4 E 0 ). When ME‐1 cells were cultured in serum‐free medium, they stopped proliferating and began to differentiate morphologically, functionally and phenotypically to mature granulocyte‐like cells. The protein kinase inhibitor, 1‐(5‐isoquinolinyl‐sulphonyl)‐2‐methylpiperazine (H‐7) enhanced this differentiation dose‐dependently. Upon addition of fetal calf serum (FCS) to the serum‐free medium, the differentiation of ME‐1 cells into granulocyte‐like cells was inhibited and they resumed cell growth. We have recently reported that the differentiation of ME‐1 cells into macrophage‐like cells induced by IL‐3 and GM‐CSF involved the activation of protein kinase C. The present results indicate that ME‐1 is a bipotential cell line that can differentiate into granulocyte‐like cells or macrophage‐like cells, and that protein kinase C is closely related to each form of differentiation.