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A reversed activity staining procedure for detection of an acquired antibody against factor XIII in a girl with factor XIII deficiency
Author(s) -
Seiving Birgitta,
Henriksson Per,
Stenberg Pål,
Nilsson Inga Marie
Publication year - 1992
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1992.tb06438.x
Subject(s) - factor xiii , chemistry , antibody , staining , microbiology and biotechnology , agarose , agarose gel electrophoresis , sepharose , biochemistry , medicine , immunology , biology , enzyme , pathology , fibrinogen , gene
S ummary . A new method for detection of inhibitors against factor XIII is described. Agarose gel electrophoresis with normal platelet poor plasma as a factor XIII source included in the gel was combined with reversed activity staining. The procedure was applied to a 10‐year‐old girl with factor XIII deficiency where substitution therapy had failed. Plasma samples from the patient inhibited the factor XIII catalysed incorporation of the fluorescent monodansylthiacadaverine into casein at a position corresponding to the migration of γ‐globulins. Treatment with Sepharose‐bound protein A effectively adsorbed the factor XIII antibody in the patient's plasma.