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Interleukin‐6 and Interleukin‐1 enhancement of GM‐CSF‐dependent proliferation of haematopoietic progenitor cells in myelodysplastic syndromes
Author(s) -
Schipperus Martin R.,
Sonneveld Pieter,
Lindemans Jan,
Lom Kirsten,
Vlastuin Margreet,
Abels Johannes
Publication year - 1991
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1991.tb08619.x
Subject(s) - haematopoiesis , progenitor cell , interleukin 3 , immunology , cancer research , medicine , interleukin , progenitor , stem cell , biology , cytokine , t cell , microbiology and biotechnology , interleukin 21 , immune system
S ummary . Interleukin‐1 (Il–1) and Interleukin‐6 (Il–6) have been reported to enhance the growth factor dependent colony formation of normal primitive haematopoietic progenitor cells as well as of leukaemic blast‐cell progenitors. We investigated the effects of Il–1β and Il–6 in combination with granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) on the in vitro colony formation of myeloid progenitors from 23 patients with a myelodysplastic syndrome (MDS). Neither Il–1β nor Il–6 were found to have colony stimulating activity on their own. In normal bone marrow cultures, either stimulated with optimal or suboptimal doses of GM‐CSF, no enhancing or antagonistic effect of Il–6 or Il–1β was detected. In a majority of the MDS cases, however, an enhancing effect of Il–6 and Il–1β in combination with GM‐CSF was observed (20/23 and 10/21 cases respectively). In three cases of the Il‐6 and GM‐CSF combination an antagonistic effect was observed as well as in four cases of the Il‐1β and GM‐CSF combination. A delayed addition of Il‐6 to the cultures did not result in an abrogation of the effect, indicating that Il‐6 is not required immediately at the initiation of the culture. These results indicate that co‐stimulation with IL–6 or IL–1β is able to augment the GM‐CSF activity on MDS myeloid progenitor cells.