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Multiparameter flow‐cytometrical quantitation of circulating CD34 + ‐cells: correlation to the quantitation of circulating haemopoietic progenitor cells by in vitro colony‐assay
Author(s) -
Serke Stefan,
Säuberlich Sabine,
Huhn Dieter
Publication year - 1991
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1991.tb08609.x
Subject(s) - cd34 , progenitor cell , flow cytometry , in vitro , immunology , haematopoiesis , biology , microbiology and biotechnology , stem cell , biochemistry
S ummary . A multiparameter flow‐cytometrical method for the quantitation of CD34 + ‐cells present in adult human peripheral blood cells (PBMC) has been developed. PBMC from 13 healthy adult subjects were analysed for CD34 + ‐cells by flow‐cytometry, with only the lymphoid population of cells negative for anti‐CD3‐moAb included in the analysis. At the same time mononuclear cells from the same individuals were depleted of CD3 + ‐ and CDl4 + ‐cells by immunomagnetic purging. These cells were cultured for haemopoietic colonies. A correlation coefficient of 0·92 was calculated by regression analysis of CD34 + ‐cells number versus the numbers of colonies (CFU‐GM, BFU‐E). Purging with anti‐CD34‐moAb abrogated colony‐growth. The flow‐cytometrical method for the determination of circulating CD34 + ‐cells was used for the determination of circulating haemopoietic progenitor cells in one patient, in order to time leukapheresis for subsequent autologous reinfusion following high‐dose chemotherapy. Determination of CD34 + ‐cells from human adult peripheral blood by multiparameter flow‐cytometry promises to be a useful tool for monitoring circulating haemopoietic progenitor cells as identified by anti‐CD34‐moAb.