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An in‐vitro direct chemiluminescence assay for assessment of platelet‐bound antibody in thrombocytopenic patients
Author(s) -
Kazemi A.,
Singh A. K.,
Slater N. G. P.
Publication year - 1991
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1991.tb08091.x
Subject(s) - thrombocytopenic purpura , platelet , medicine , immunology , immune system , antibody , chemiluminescence , immune thrombocytopenia , immunofluorescence , in vitro , gastroenterology , biology , chemistry , biochemistry , organic chemistry
A direct chemiluminescence test (CLT) employing autologous monocytes and granulocytes was used to assay platelet‐bound antibody in comparison to the direct platelet suspension immunofluorescence test (PSIFT) in 50 patients. Twenty‐six of the 30 immune thrombocytopenic purpura (ITP) patients, including all 21 chronic ITP patients with platelet counts below 100 × 10 9 /1, showed positive results with at least one type of phagocytic cell; so did seven of the eight patients with other presumed autoimmune thrombocytopenias, and three out of 12 patients with thrombocytopenia associated with non‐immune disorders. This test appears potentially valuable in the clinical diagnosis of immune thrombocytopenias.