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Non‐radioactive detection of immunoglobulin and T cell receptor gene rearrangement in acute lymphoblastic leukaemia
Author(s) -
Martin Gerald,
Lawlor Emer
Publication year - 1991
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1991.tb08065.x
Subject(s) - gene rearrangement , t cell receptor , microbiology and biotechnology , gene , antibody , immunoglobulin heavy chain , acute lymphocytic leukemia , biology , dna , t cell , leukemia , immunology , genetics , immune system , lymphoblastic leukemia
Rearrangements of the heavy chain immunoglobulin gene and T cell receptor β gene were investigated in 25 patients suffering from precursor B cell acute leukaemia and six patients suffering from T cell acute leukaemia using biotinylated DNA probes. All precursor B acute leukaemia patients had IgH gene rearrangements and 63% of those studied also had TCRβ gene rearrangements. All T cell acute leukaemia patients had TCRβ gene rearrangements and germline IgH configuration. Dilution experiments indicated that DNA from leukaemic cells representing 1–2% of a tested sample could be detected using this technique which compares favourably to radioactive DNA probes.