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Absence of phosphatidylinositol (PI)‐linked proteins in a very early human multipotential haematopoietic marrow cell
Author(s) -
Huang Andrew T.,
Panella Timothy J.,
Mold Nelda G.,
Rosse Wendell F.
Publication year - 1991
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1991.tb07969.x
Subject(s) - haematopoiesis , biology , progenitor cell , population , cd34 , stem cell , decay accelerating factor , microbiology and biotechnology , bone marrow , immunology , antibody , complement system , medicine , environmental health
Summary A very early human haematopoietic progenitor cell population which was negative for the major histocompatibility class II antigen (HLA‐DR) and positive for the CD34 (MY10) antigen was separated into two subsets according to the expression of decay‐accelerating factor (DAF) on the cell surface. Using immunoadherence, cell cycle analysis, and cell culture, we determined that there is a DAF − multipotential cell and a more differentiated DAF + lineage specific progenitor cell existing in human bone marrow. The DAF + subset was highly enriched for CFU‐GEMM, while the DAF + subset contained only BFU‐E and CFU‐GM. The DAF − subset was approximately 0·03% and the DAF + subset approximately 0·0·08% of the original bone marrow population. MIRL (membrane‐inhibitory of reactive lysis), another Pl‐linked protein, was not expressed on the DAF − population but was expressed on the DAF + cells. These observations indicate that PI‐linked proteins are absent from the multipotential stem cell but are present on an early lineage specific cell. The absence of expression of PI‐linked proteins can be used to further isolate and characterize a very early multipotential haematopoietic progenitor cell population.