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Interleukin 6, a possible autocrine growth and differentiation factor for the human megakaryocytic cell line, CMK
Author(s) -
Fuse Akira,
Kakuda Harumi,
Shima Yukichi,
Van Damme Jo,
And Alfons Billiau†,
Sato Takeyuki
Publication year - 1991
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1991.tb07944.x
Subject(s) - autocrine signalling , cell culture , cytokine , microbiology and biotechnology , growth factor , cell growth , glycoprotein , biology , cell , cell division , chemistry , immunology , biochemistry , genetics , receptor
Summary. CMK is a human cell line derived from a megakaryoblastic leukaemia. It has characteristics of the megakaryocytic lineage, such as the presence of platelet peroxidase, membrane glycoproteins (GP)Ib and GPIIb/IIIa, α‐granules, and demarcation membranes. The cell line proliferates autonomously in serum‐containing medium. Here we report that the cell line expresses the gene for IL‐6 and releases small quantities of the cytokine into the medium. Addition of exogenous IL‐6 to cultures seeded into medium was found to promote growth of the cells. Conversely, addition of a neutralizing anti‐IL‐6 antibody inhibited cell growth. These data support the notion that autocrine IL‐6 is one of the factors accounting for autonomous growth of the cell line.