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Inactivation of the retinoblastoma susceptibility gene in a human high grade non‐Hodgkin's lymphoma cell line
Author(s) -
Weide R.,
Dowding C.,
Sucai Bi,
Bungey J.,
Chase A.,
Goldman J. M.
Publication year - 1991
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1991.tb04479.x
Subject(s) - retinoblastoma , lymphoma , blot , southern blot , biology , karyotype , microbiology and biotechnology , allele , cell culture , tumor suppressor gene , gene , loss of heterozygosity , northern blot , cancer research , cytogenetics , chromosome , gene expression , genetics , carcinogenesis , immunology
Summary We studied the expression of the retinoblastoma (RB) gene product (p105) in a B‐cell line established from a patient with non‐Hodgkin's lymphoma (large cell type). The karyotype of this cell line, named Ri‐1, showed amongst other changes an apparent deletion of one chromosome 13 on band q14. No p105 could be detected by immunoprecipitation analysis and Western blotting in Ri‐1 cells. Northern blotting revealed that RB mRNA is not expressed in Ri‐1. Southern blotting confirmed the loss of one RB allele but showed a normal gross structure of the remaining allele. This suggests that the inactivation of the RB gene in Ri‐1 cells is due to deletion of one allele and point mutations or small deletions in the other, as is often the case in retinoblastomas. Our findings imply that inactivation of the RB gene may play a role in the pathogenesis of high grade malignant lymphomas and that studies of RB in primary lymphoma samples would be of interest.