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Interleukin‐1 is one factor which regulates autocrine production of GM‐CSF by the blast cells of acute myeloblastic leukaemia
Author(s) -
Bradbury D.,
Rogers S.,
Kozlowski R.,
Bowen G.,
Reilly I. A. G.,
Russell N. H.
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb07905.x
Subject(s) - autocrine signalling , acute myeloblastic leukemia , cancer research , medicine , immunology , leukemia , receptor
S ummary The role of interleukin‐1 (IL‐1) as a regulator of the autonomous growth of the blast cells of acute myeloid leukaemia (AML) has been studied on samples isolated from 15 patients. In nine out of 10 patients with evidence of partial autonomous blast cell growth. IL‐1 further stimulated cell growth in both suspension culture and in a clonogenic assay. 11,‐1 also stimulated cell growth in two out of three cases with no evidence of autonomous growth whereas no additional stimulation was observed in two cases with totally autonomous growth. In blast cells stimulated by IL‐1, synthesis of granulocyte‐macrophage colony stimulating factor (GM‐CSF) was increased and the proliferative response to IL‐1 was inhibited by an antibody to GM‐CSF. In all samples with evidence of autonomous growth, blast cell conditioned medium (BCCM) contained IL‐1 activity (range 0.7–74.2 units/ml) and a polyclonal antibody to IL‐1 markedly inhibited autonomous growth in four samples. BCCM from three of these four samples contained GM‐CSF, the synthesis of which was suppressed when BCCM was prepared in the presence of anti‐IL‐1. Our data suggests that endogenous IL‐1 is an important factor in the regulation of the production of GM‐CSF and hence of autonomous growth of AML blasts, but that other mechanisms regulating GM‐CSF production may exist.