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Erythropoietin production in kidney tubular cells
Author(s) -
Maxwell Alexander P.,
Lappin Terence R. J.,
Johnston Colin F.,
Bridges John M.,
McGeown Mary G.
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb06347.x
Subject(s) - erythropoietin , recombinant dna , in situ hybridization , gene , messenger rna , biology , oligonucleotide , kidney , gene expression , microbiology and biotechnology , immunohistochemistry , antibody , immunology , genetics
Summary The erythropoietin gene has been cloned in three mammalian species including man and recombinant erythropoietin is now used to treat the anaemia of chronic renal failure. Despite the isolation of the gene the precise cellular location of erythropoietin synthesis remains controversial. We present studies which demonstrate erythropoietin production by kidney tubular cells. Erythropoietin gene expression (messenger RNA) was detected by in situ hybridization using an oligonucleotide gene probe and the translated protein product by immunohistochemistry employing antibodies raised to pure recombinant DNA derived erythropoietin.