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Acute ‘bilineal‐biphenotypic’ leukaemia
Author(s) -
Akashi Koichi,
Shibuya Tsunefumi,
Harada Mine,
Morioka Eiji,
Oshima Koichi,
Kimura Nobuhiro,
Takeshita Morishige,
Kurokawa Mamoru,
Kikuchi Masahiro,
Niho Yoshiyuki
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb06326.x
Subject(s) - cd15 , bone marrow , biology , pathology , population , immunofluorescence , lymph node , karyotype , lymphoblast , microbiology and biotechnology , medicine , immunology , cell culture , gene , antibody , genetics , chromosome , cd34 , stem cell , environmental health
Summary We describe a unique case of hybrid leukaemia with bilineal and biphenotypic features. The coexistence of lymphoblasts and monoblasts was determined morphologically and cytochemically. Immunofluorescence and immunohistochemical analysis revealed that each blast population had both T lymphoid (CD2, cytoplasmic CD3) and myeloid (CD11, CD13, CD15) markers. Southern blot analysis of DNA extracted from the lymph node biopsy demonstrated the presence of monoclonal rearrangement of the TcR‐Cβ gene. Cytogenetic analysis of the bone marrow cells showed a karyotype of 48, XY, 7q + in all of the metaphases examined. These observations are suggestive of a monoclonal origin for these two distinct blast populations.