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Characterization of the erythropoietic progenitor cells (BFU‐E) in paroxysmal nocturnal haemoglobinuria (PNH)
Author(s) -
Terasawa Takashi,
Shichishima Tsutomu,
Matsuda Minoru,
Uchida Tatsumi,
Kariyone Shigeo
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb04359.x
Subject(s) - erythropoiesis , population , trypan blue , progenitor cell , lysis , erythroblast , immunology , biology , cell , microbiology and biotechnology , stem cell , medicine , genetics , anemia , environmental health
Summary. In order to investigate erythropoiesis in paroxysmal nocturnal haemoglobinuria (PNH), the relative proportions of complement‐mediated lysed erythroblasts in individual bursts of two cases of PNH were analysed by a modified complement lysis sensitivity test using the trypan blue dye exclusion method. All bursts contained both complement‐sensitive and complement‐insensitive erythroblasts and the ratio between them varied from burst to burst. The means ±SD of the haemolytic percentages of individual bursts in cases 1 and 2 were 30.9 ± 10.8% ( n = 20, ranging from 13.2% to 52.8%) and 54.8 ± 16.4% ( n = 31, ranging from 26.4% to 82.5%), respectively. In addition, the cumulative frequency distributions of the proportions of complement‐mediated lysed erythroblasts in individual bursts approached normal frequency distributions. These results suggest that two red cell populations with different susceptibilities to complement in PNH are derived from a single population of erythropoietic progenitor cells rather than a dual population of normal and PNH erythropoietic progenitor cells.